EIF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93312

Novus Biologicals, part of Bio-Techne
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NB100-93312
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all EIF5 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 5 using the numbering given in entry NP_892116.2 (GeneID 1983).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF5 Antibody

Western Blot: EIF5 Antibody [NB100-93312]

Western Blot: EIF5 Antibody [NB100-93312]

Western Blot: EIF5 Antibody [NB100-93312] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used for WB at 0.04 ug/ml.

Applications for EIF5 Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
This antibody is useful for western blot. It is not recommended for immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EIF5

Eukaryotic initiation factor 5 (eIF5) plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, eIF5 interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. eIF5 is also reported to form a complex with eIF2. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.

Alternate Names

eIF-5, EIF-5A, eukaryotic translation initiation factor 5

Entrez Gene IDs

1983 (Human); 217869 (Mouse)

Gene Symbol

EIF5

UniProt

NP_892116.2 (Human)

Additional EIF5 Products

Product Documents for EIF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EIF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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