EIF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86184

Novus Biologicals, part of Bio-Techne
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NBP1-86184
NBP1-86184-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EIF5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF5 Antibody

Western Blot: EIF5 Antibody [NBP1-86184]

Western Blot: EIF5 Antibody [NBP1-86184]

Western Blot: EIF5 Antibody [NBP1-86184] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: EIF5 Antibody [NBP1-86184]

Immunocytochemistry/ Immunofluorescence: EIF5 Antibody [NBP1-86184]

Immunocytochemistry/Immunofluorescence: EIF5 Antibody [NBP1-86184] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: EIF5 Antibody [NBP1-86184]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP1-86184]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP1-86184] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
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Applications for EIF5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF5

EIF5 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingos

Alternate Names

eIF-5, EIF-5A, eukaryotic translation initiation factor 5

Gene Symbol

EIF5

Additional EIF5 Products

Product Documents for EIF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EIF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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