EIF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00231

Novus Biologicals, part of Bio-Techne
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NBP3-00231-100ul
NBP3-00231-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EIF5 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human EIF5 (Accession#: P55010; Met1-Asp150)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EIF5 Antibody

Immunocytochemistry/ Immunofluorescence: EIF5 Antibody [NBP3-00231]

Immunocytochemistry/ Immunofluorescence: EIF5 Antibody [NBP3-00231]

Immunocytochemistry/Immunofluorescence: EIF5 Antibody [NBP3-00231] - Immunofluorescence staining of EIF5 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human EIF5 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231] - Immunochemical staining of human EIF5 in human colon carcinoma with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231]

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP3-00231] - Immunochemical staining of human EIF5 in human breast carcinoma with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).

Applications for EIF5 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:100-1:500

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF5

EIF5 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingos

Alternate Names

eIF-5, EIF-5A, eukaryotic translation initiation factor 5

Gene Symbol

EIF5

Additional EIF5 Products

Product Documents for EIF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EIF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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