eIF5A Antibody (5E1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26604
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5E1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human eIF5A [UniProt P63241]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for eIF5A Antibody (5E1)
Immunohistochemistry: eIF5A Antibody (5E1) [NBP3-26604] -
Immunohistochemistry: eIF5A Antibody (5E1) [NBP3-26604] - Image of eIF5A Antibody (5E1) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: eIF5A Antibody (5E1) [NBP3-26604] -
Western Blot: eIF5A Antibody (5E1) [NBP3-26604] - Positive Western Blot detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate.All lanes: eIF5A Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 17, 21 kDa
Observed band size: 18 kDa
Immunocytochemistry/Immunofluorescence: eIF5A Antibody (5E1) [NBP3-26604] -
Immunocytochemistry/Immunofluorescence: eIF5A Antibody (5E1) [NBP3-26604] - Staining of Hela Cells with eIF5A Antibody (5E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for eIF5A Antibody (5E1)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: eIF5A
Long Name
Eukaryotic Translation Initiation Factor 5A
Alternate Names
eIF4D, Rev-binding Factor
Gene Symbol
EIF5A
Additional eIF5A Products
Product Documents for eIF5A Antibody (5E1)
Product Specific Notices for eIF5A Antibody (5E1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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