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eIF5A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24814

Novus Biologicals, part of Bio-Techne
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NBP3-24814

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein eIF5A using the following amino acid sequence: EGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF5A Antibody

eIF5A Antibody Immunocytochemistry/Immunofluorescence: eIF5A Antibody [NBP3-24814]

Immunocytochemistry/Immunofluorescence: eIF5A Antibody [NBP3-24814]

Staining of human cell line U2OS shows localization to cytosol.

Applications for eIF5A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF5A

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Long Name

Eukaryotic Translation Initiation Factor 5A

Alternate Names

eIF4D, Rev-binding Factor

Gene Symbol

EIF5A

Additional eIF5A Products

Product Documents for eIF5A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF5A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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