eIF5A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85943

Novus Biologicals, part of Bio-Techne
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NBP1-85943
NBP1-85943-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF5A2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IKRNDYQLICIQDGYLSLLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQVSVMCAMSEEYAVA

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24484057).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF5A2 Antibody

Western Blot: eIF5A2 Antibody [NBP1-85943]

Western Blot: eIF5A2 Antibody [NBP1-85943]

Western Blot: eIF5A2 Antibody [NBP1-85943] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: eIF5A2 Antibody [NBP1-85943]

Immunocytochemistry/ Immunofluorescence: eIF5A2 Antibody [NBP1-85943]

Immunocytochemistry/Immunofluorescence: eIF5A2 Antibody [NBP1-85943] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943]

Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943]

Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human kidney shows moderate positivity in nuclear membrane in cells in tubules.
View 4 more images

Applications for eIF5A2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24484057). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF5A2

Eukaryotic translation initiation factor 5A (eIF5A) is an essential protein tightly linked to cellular polyamine homeostasis. EIF5A2, a novel phylogenetically conserved gene for eIF5A islocalized in an area of the genome often noted for chromosomal instability in cancers. EIF5A2 expression has been observed in testis, brain and colorectal adenocarcinoma

Alternate Names

EIF-5A2, eIF-5A-2, eIF5AII, eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 2, eukaryotic translation initiation factor 5A2, eukaryotic translation initiation factor 5A-2

Gene Symbol

EIF5A2

Additional eIF5A2 Products

Product Documents for eIF5A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF5A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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