Elastin Antibody (ELN/1981)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75767
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # ELN/1981
Concentration
0.2 mg/ml
Product Summary for Elastin Antibody (ELN/1981)
Immunogen
Recombinant full-length human Elastin protein (Uniprot: P15502)
Localization
Secreted and Extracellular Space
Marker
Marker of Arterial Stiffness and Atherosclerosis
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75770)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Elastin Antibody (ELN/1981)
Immunohistochemistry-Paraffin: Elastin Antibody (ELN/1981) [NBP2-75767]
Immunohistochemistry-Paraffin: Elastin Antibody (ELN/1981) [NBP2-75767] - Formalin-fixed, paraffin-embedded human Small Intestine stained with Monospecific Mouse Monoclonal Antibody (ELN/1981) to Elastin.Protein Array: Elastin Antibody (ELN/1981) [NBP2-75767]
Protein Array: Elastin Antibody (ELN/1981) [NBP2-75767] - Analysis of Protein Array containing >19,000 full-length human proteins using Elastin Antibody (ELN/1981) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.Applications for Elastin Antibody (ELN/1981)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Elastin
Alternate Names
elastin, FLJ38671, FLJ43523, SVAS, tropoelastin, WBS, WS
Gene Symbol
ELN
UniProt
Additional Elastin Products
Product Documents for Elastin Antibody (ELN/1981)
Product Specific Notices for Elastin Antibody (ELN/1981)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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