ELF3/ESE-1 Antibody (7O2V4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16429
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7O2V4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ELF3/ESE-1 Antibody (7O2V4)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-371 of human ELF3/ESE-1 (P78545). MAATCEISNIFSNYFSAMYSSEDSTLASVPPAATFGADDLVLTLSNPQMSLEGTEKASWLGEQPQFWSKTQVLDWISYQVEKNKYDASAIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWIIELLEKDGMAFQEALDPGPFDQGSPFAQELLDDGQQASPYHPGSCGAGAPSPGSSDVSTAGTGASRSSHSSDSGGSDVDLDPTDGKLFPSDGFRDCKKGDPKHGKRKRGRPRKLSKEYWDCLEGKKSKHAPRGTHLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRLVYKFGKNSSGWKEEEVLQSRN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ELF3/ESE-1 Antibody (7O2V4)
![Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429]](https://resources.bio-techne.com/images/products/ELF3-ESE-1-Antibody-7O2V4-Western-Blot-NBP3-16429-img0001.jpg "Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429]")
Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429]
Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Western blot analysis of extracts of various cell lines, using ELF3/ESE-1 Rabbit mAb (NBP3-16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -
Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Analysis of extracts of HepG2 cells, using ESE1/ELF3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chip IP Sequencing: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -
Chip IP Sequencing: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HepG5 with 1μg ESE1 along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (MARK4), as shown in figure.Applications for ELF3/ESE-1 Antibody (7O2V4)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5μg antibody for 10μg-15μg of Chromatin
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Western Blot
1:500 - 1:2000
Application Notes
CUT&Tag, 10 cells /1 ug
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ELF3
Long Name
E74-like factor 3
Alternate Names
EPR1, ERT, ESX, JEN
Gene Symbol
ELF3
Additional ELF3 Products
Product Documents for ELF3/ESE-1 Antibody (7O2V4)
Product Specific Notices for ELF3/ESE-1 Antibody (7O2V4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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