Elongin A Antibody (1F3)

Novus Biologicals, part of Bio-Techne | Catalog # H00006924-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Elongin A Antibodies »

Product Specifications

Immunogen

TCEB3 (NP_003189, 81 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NAEPDEQDFEKSNSRKRPRDALQKEEEMEGDYQETWKATGSRSYSPDHRQKKHRKLSELERPHKVSHGHERRDERKRCHRMSPTYSSDPESSDYGHVQSPPSCTSPHQMY

Specificity

TCEB3 (1F3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Elongin A Antibody (1F3)

Western Blot: Elongin A Antibody (1F3) [H00006924-M02]

Western Blot: Elongin A Antibody (1F3) [H00006924-M02]

Western Blot: Elongin A Antibody (1F3) [H00006924-M02] - TCEB3 monoclonal antibody (M02), clone 1F3 Analysis of TCEB3 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: Elongin A Antibody (1F3) [H00006924-M02]

Immunocytochemistry/ Immunofluorescence: Elongin A Antibody (1F3) [H00006924-M02]

Immunocytochemistry/Immunofluorescence: Elongin A Antibody (1F3) [H00006924-M02] - Analysis of monoclonal antibody to TCEB3 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Elongin A Antibody (1F3) [H00006924-M02]

Western Blot: Elongin A Antibody (1F3) [H00006924-M02]

Western Blot: Elongin A Antibody (1F3) [H00006924-M02] - Analysis of TCEB3 expression in transfected 293T cell line by TCEB3 monoclonal antibody (M02), clone 1F3.Lane 1: TCEB3 transfected lysate(87.2 KDa).Lane 2: Non-transfected lysate.

Applications for Elongin A Antibody (1F3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Elongin A

This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Alternate Names

EloA, Elongin 110 kDa subunit, elongin-A, FLJ38760, FLJ42849, RNA polymerase II transcription factor SIII subunit A1, SIII, SIII p110, TCEB3A, transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A), transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A), transcription elongation factor B alpha subunit, transcription elongation factor B polypeptide 3

Entrez Gene IDs

6924 (Human)

Gene Symbol

TCEB3

OMIM

600786 (Human)

UniProt

NP_003189 (Human)

Additional Elongin A Products

Product Documents for Elongin A Antibody (1F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Elongin A Antibody (1F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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