Elp3 Antibody (3R3U5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15245
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3R3U5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Elp3 Antibody (3R3U5)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-547 of human Elp3 (Q9H9T3). MRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRKVLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSERSLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFKVVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVARILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQVELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPTKFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Elp3 Antibody (3R3U5)
![Western Blot: Elp3 Antibody (3R3U5) [NBP3-15245]](https://resources.bio-techne.com/images/products/Elp3-Antibody-3R3U5-Western-Blot-NBP3-15245-img0003.jpg "Western Blot: Elp3 Antibody (3R3U5) [NBP3-15245]")
Western Blot: Elp3 Antibody (3R3U5) [NBP3-15245]
Western Blot: Elp3 Antibody (3R3U5) [NBP3-15245] - Western blot analysis of extracts of various cell lines, using Elp3 Rabbit mAb (NBP3-15245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245]](https://resources.bio-techne.com/images/products/Elp3-Antibody-3R3U5-Immunohistochemistry-Paraffin-NBP3-15245-img0002.jpg "Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245]")
Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245]
Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245] - Immunohistochemistry of paraffin-embedded mouse testis using Elp3 Rabbit mAb (NBP3-15245) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245]](https://resources.bio-techne.com/images/products/Elp3-Antibody-3R3U5-Immunohistochemistry-Paraffin-NBP3-15245-img0001.jpg "Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245]")
Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245]
Immunohistochemistry-Paraffin: Elp3 Antibody (3R3U5) [NBP3-15245] - Immunohistochemistry of paraffin-embedded rat brain using Elp3 Rabbit mAb (NBP3-15245) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Elp3 Antibody (3R3U5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Elp3
Alternate Names
EC 2.3.1.48, elongation protein 3 homolog (S. cerevisiae), elongator complex protein 3, hELP3, KAT9FLJ10422
Gene Symbol
ELP3
Additional Elp3 Products
Product Documents for Elp3 Antibody (3R3U5)
Product Specific Notices for Elp3 Antibody (3R3U5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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