EME1 Antibody (MTA31 7H2/1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08496
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MTA31 7H2/1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
His-tagged recombinant Eme1 of human origin (Uniprot: Q96AY2)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07779).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for EME1 Antibody (MTA31 7H2/1) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug of protein 1ml of lysate
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: EME1
Alternate Names
crossover junction endonuclease EME1, EC 3.1.22, EC 3.1.22.-, essential meiotic endonuclease 1 homolog 1 (S. pombe), essential meiotic endonuclease 1 homolog 2, FLJ31364, hMMS4, homolog of yeast EME1 endonuclease, MMS4, MMS4 homolog, MMS4L, SLX2 structure-specific endonuclease subunit homolog A, SLX2A
Gene Symbol
EME1
Additional EME1 Products
Product Documents for EME1 Antibody (MTA31 7H2/1) - Azide and BSA Free
Product Specific Notices for EME1 Antibody (MTA31 7H2/1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...