Emerin Antibody (10Y5K9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16351
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10Y5K9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-254 of human Emerin (P50402). RERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Emerin Antibody (10Y5K9)
Western Blot: Emerin Antibody (10Y5K9) [NBP3-16351]
Western Blot: Emerin Antibody (10Y5K9) [NBP3-16351] - Western blot analysis of extracts of various cell lines, using Emerin Rabbit mAb (NBP3-16351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunocytochemistry/ Immunofluorescence: Emerin Antibody (10Y5K9) [NBP3-16351]
Immunocytochemistry/Immunofluorescence: Emerin Antibody (10Y5K9) [NBP3-16351] - Immunofluorescence analysis of NIH-3T3 cells using Emerin Rabbit mAb (NBP3-16351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: Emerin Antibody (10Y5K9) [NBP3-16351]
Immunohistochemistry-Paraffin: Emerin Antibody (10Y5K9) [NBP3-16351] - Immunohistochemistry of paraffin-embedded human colon using Emerin Rabbit mAb (NBP3-16351) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Emerin Antibody (10Y5K9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Emerin
Alternate Names
emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5
Gene Symbol
EMD
Additional Emerin Products
Product Documents for Emerin Antibody (10Y5K9)
Product Specific Notices for Emerin Antibody (10Y5K9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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