Emerin Antibody (10Y5K9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16351

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16351-100ul
NBP3-16351-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10Y5K9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Emerin Antibodies »

Product Summary for Emerin Antibody (10Y5K9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-254 of human Emerin (P50402). RERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Emerin Antibody (10Y5K9)

Western Blot: Emerin Antibody (10Y5K9) [NBP3-16351]

Western Blot: Emerin Antibody (10Y5K9) [NBP3-16351]

Western Blot: Emerin Antibody (10Y5K9) [NBP3-16351] - Western blot analysis of extracts of various cell lines, using Emerin Rabbit mAb (NBP3-16351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (10Y5K9) [NBP3-16351]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (10Y5K9) [NBP3-16351]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (10Y5K9) [NBP3-16351] - Immunofluorescence analysis of NIH-3T3 cells using Emerin Rabbit mAb (NBP3-16351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Emerin Antibody (10Y5K9) [NBP3-16351]

Immunohistochemistry-Paraffin: Emerin Antibody (10Y5K9) [NBP3-16351]

Immunohistochemistry-Paraffin: Emerin Antibody (10Y5K9) [NBP3-16351] - Immunohistochemistry of paraffin-embedded human colon using Emerin Rabbit mAb (NBP3-16351) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Emerin Antibody (10Y5K9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Emerin

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Alternate Names

emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5

Gene Symbol

EMD

Additional Emerin Products

Product Documents for Emerin Antibody (10Y5K9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Emerin Antibody (10Y5K9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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