Emerin Antibody (CL0203)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52877

Novus Biologicals, part of Bio-Techne
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NBP2-52877
NBP2-52877-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0203

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Emerin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY

Epitope

QSKGYNDDYY

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Emerin Antibody (CL0203)

Western Blot: Emerin Antibody (CL0203) [NBP2-52877]

Western Blot: Emerin Antibody (CL0203) [NBP2-52877]

Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Analysis of extracts from RT-4 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-EMD monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/ Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877]

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877]

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human testis shows nuclear membrane immunoreactivity.
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Applications for Emerin Antibody (CL0203)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Emerin

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Alternate Names

emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5

Gene Symbol

EMD

Additional Emerin Products

Product Documents for Emerin Antibody (CL0203)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Emerin Antibody (CL0203)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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