Emerin Antibody (EMD/2167)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79784
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]](https://resources.bio-techne.com/images/products/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0002.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]")
![Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784]](https://resources.bio-techne.com/images/products/Emerin-Antibody-EMD-2167-Western-Blot-NBP2-79784-img0006.jpg "Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # EMD/2167
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary) (Uniprot: P50402)
Localization
Nuclear Membrane
Specificity
Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.
Marker
Papillary Thyroid Carcinoma and EDMD Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79918)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Emerin Antibody (EMD/2167)
Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784]
Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784] - Western Blot Analysis of human HeLa cell lysate using Emerin Antibody (EMD/2167).![Immunocytochemistry/ Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784]](https://resources.bio-techne.com/images/products/Emerin-Antibody-EMD-2167-Immunocytochemistry-Immunofluorescence-NBP2-79784-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784]")
Immunocytochemistry/ Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784]
Immunocytochemistry/Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784] - Immunofluorescence Analysis of Human HeLa cells labeling Emerin with Emerin Antibody (EMD/2167) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]](https://resources.bio-techne.com/images/products/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0004.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]")
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Antibody (EMD/2167).![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]](https://resources.bio-techne.com/images/products/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0003.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784]")
![Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784]](https://resources.bio-techne.com/images/products/Emerin-Antibody-EMD-2167-Protein-Array-NBP2-79784-img0005.jpg "Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784]")
Applications for Emerin Antibody (EMD/2167)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-3 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Emerin
Alternate Names
emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5
Gene Symbol
EMD
UniProt
Additional Emerin Products
Product Documents for Emerin Antibody (EMD/2167)
Product Specific Notices for Emerin Antibody (EMD/2167)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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