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EMILIN1 Antibody (C11A8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36338

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # C11A8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

recombinant mouse Emilin-1

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for EMILIN1 Antibody (C11A8) - Azide and BSA Free

Western Blot: EMILIN1 Antibody (C11A8)Azide and BSA Free [NBP2-36338]

Western Blot: EMILIN1 Antibody (C11A8)Azide and BSA Free [NBP2-36338]

Western Blot: EMILIN1 Antibody (C11A8) [NBP2-36338] - Western blot on recombinant protein obtained from supernatant of cells expressing human EMILIN1 (hE1), mouse EMILIN1 (mE1), mouse EMILIN2 (mE2).
Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/ Immunofluorescence: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunocytochemistry/Immunofluorescence: EMILIN1 Antibody (C11A8) [NBP2-36338] - Positive staining of EMILIN1 (green) in mouse lymphatic endothelial cells (LAEC). Nuclei are stained in blue. Scale bar 38 um.
Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) - Azide and BSA Free [NBP2-36338]

Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) [NBP2-36338] - EMILIN1 is expressed in association with lymphatic vessels. Cryostat sections of normal mouse tissues stained with anti-EMILIN1 (green) antibodies. In all mouse tissues and organs examined, EMILIN1 was uniformly distributed in the stroma. In the skin, EMILIN1 staining colocalizes with LYVE-1-positive lymphatic vessels surrounding hair follicles. In the small intestine, EMILIN1 colocalizes with LYVE-1-positive lacteals and submucosal lymphatic vessels. At higher magnification, in the lung and lymph nodes, it is more evident that EMILIN1 is distributed at the abluminal surfaces of LECs . In the lymph node, EMILIN1-positive fibers connecting LECs to the surrounding ECM are evident.

Applications for EMILIN1 Antibody (C11A8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

2-10 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMILIN1

The EMILIN1 gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interfacedomain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encodedprotein associates with

Alternate Names

DKFZp586M121, elastin microfibril interface-located protein 1, elastin microfibril interfacer 1, EMI, EMILIN, EMILIN-1, gp115

Gene Symbol

EMILIN1

Additional EMILIN1 Products

Product Documents for EMILIN1 Antibody (C11A8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EMILIN1 Antibody (C11A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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