Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all EML4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 931 and 981of human echinoderm microtubule associated protein like 4 using the numbering given in entry NP_061936.2 (GeneID 27436).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EML4 Antibody

Western Blot: EML4 Antibody [NBP1-05974]

Western Blot: EML4 Antibody [NBP1-05974]

Western Blot: EML4 Antibody [NBP1-05974] - Whole cell lysate from HeLa cells. EML4 was also immunoprecipitated by rabbit anti-EML4 antibody NBP1-05973.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05974]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05974]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05974] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.

Applications for EML4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EML4

Echinoderm microtubule associated protein like 4 (EML4) has been identified as part of a gene fusion with the kinase domain of the anaplastic lymphoma receptor tyrosine kinase (ALK) in non-small cell lung cancer. EML4 associates with in vitro polymerized microtubules. In the cell, phosphorylated EML4 associates with the mitotic spindle and is essential for proliferation and microtubule network formation. Alternative names for EML4 include EMAPL4, ropp120, C2orf2, ELP120, and EMAP-4.

Alternate Names

C2orf2, echinoderm microtubule associated protein like 4, echinoderm microtubule-associated protein-like 4, ELP120, EMAP-4, EMAPL4, FLJ10942, FLJ32318, Restrictedly overexpressed proliferation-associated protein, Ropp 120, ROPP120DKFZp686P18118

Entrez Gene IDs

27436 (Human)

Gene Symbol

EML4

UniProt

NP_061936.2 (Human)

Additional EML4 Products

Product Documents for EML4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EML4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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