EMMPRIN/CD147 Antibody (125)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89320

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 125

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EMMPRIN/CD147 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human EMMPRIN/CD147 (Accession#: NP_940991.1; Met 1-His 205).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EMMPRIN/CD147 Antibody (125)

Western Blot: EMMPRIN/CD147 Antibody (125) [NBP2-89320]

Western Blot: EMMPRIN/CD147 Antibody (125) [NBP2-89320]

Western Blot: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDa Observed band size:54 kDa
Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320]

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320]

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of CD147 in monkey stomach with rabbit monoclonal antibody (1:1000, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
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Applications for EMMPRIN/CD147 Antibody (125)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Immunohistochemistry-Frozen

1:500-1:2000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMMPRIN/CD147

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Gene Symbol

BSG

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (125)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EMMPRIN/CD147 Antibody (125)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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