EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34543
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free [NBP2-34543]](https://resources.bio-techne.com/images/products/EMMPRIN-CD147-Antibody-8D6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34543-img0001.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free [NBP2-34543]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8D6
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human T cell line Molt 13
Localization
Cell surface
Specificity
This monoclonal antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-33015).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free [NBP2-34543]
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free [NBP2-34543] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD147 Monoclonal Antibody (8D6).Applications for EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migration through Matrigel: Order Ab without BSA/Azide.
Optimal dilution for a specific application should be determined.
Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migration through Matrigel: Order Ab without BSA/Azide.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: EMMPRIN/CD147
Long Name
Extracellular Matrix Metalloproteinase Inducer
Alternate Names
Basigin, BSG, CD147
Gene Symbol
BSG
Additional EMMPRIN/CD147 Products
Product Documents for EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free
Product Specific Notices for EMMPRIN/CD147 Antibody (8D6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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