EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-430

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Equine

Cited:

Human, Equine

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, Flow Cytometry, IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-M6/1

Format

BSA Free

Concentration

1.0 mg/ml

View all EMMPRIN/CD147 Antibodies »

Product Specifications

Immunogen

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, C2-and C3 domain of human IgG1, CD147Rg is secreted by transfectants as a dimer (NM_001728.2).

Reactivity Notes

Equine reactivity reported in scientific literature (PMID: 24998980).

Specificity

The antibody MEM-M6/1 recognizes an epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free [NB500-430]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free [NB500-430]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free [NB500-430]

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free [NB500-430]

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) purified, GAM-APC.
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free [NB500-430]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free [NB500-430]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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Applications for EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Western Blot

1 ug/ml
Application Notes
Western Blot - Non-reducing conditions. Use in ELISA reported in scientific literature (PMID 24710016). This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EMMPRIN/CD147

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Entrez Gene IDs

682 (Human)

Gene Symbol

BSG

UniProt

P35613 (Human)

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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