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EMP/MAEA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17200

Novus Biologicals, part of Bio-Techne
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NBP2-17200

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Chicken (95%), Mouse (98%), Rat (98%), Rhesus Macaque (100%), Xenopus (95%), Zebrafish (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EMP/MAEA. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Localization

Nucleus matrix; Cell membrane; Cytoplasm; cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EMP/MAEA Antibody

Western Blot: EMP/MAEA Antibody [NBP2-17200]

Western Blot: EMP/MAEA Antibody [NBP2-17200]

Western Blot: MAEA Antibody [NBP2-17200] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: EMP/MAEA Antibody [NBP2-17200]

Immunocytochemistry/ Immunofluorescence: EMP/MAEA Antibody [NBP2-17200]

Immunocytochemistry/Immunofluorescence: MAEA Antibody [NBP2-17200] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using MAEA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Applications for EMP/MAEA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EMP/MAEA

This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Long Name

Erythroblast Macrophage Protein/Macrophage Erythroblast Attacher

Alternate Names

EMLP, HLC-10, MAEA, PIG5

Gene Symbol

MAEA

UniProt

Additional EMP/MAEA Products

Product Documents for EMP/MAEA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EMP/MAEA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.