Endoglin/CD105 Antibody (MEM-229) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-58718
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0003.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]")
![Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-MEM-229-Immunofluorescence-NB110-58718-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Canine (Negative), Equine (Negative)
Cited:
Porcine
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # MEM-229
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
recombinant vaccinia virus containing human CD105 (L-isoform) cDNA
Specificity
This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Marker
Neo-endothelial Cells Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Endoglin/CD105 Antibody (MEM-229) - BSA Free
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]
Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0006.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]")
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PerCP-CyTM5.5.![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0002.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]")
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT.![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0004.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0005.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]")
![Endoglin/CD105 Antibody (MEM-229) - BSA Free Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]](https://resources.bio-techne.com/images/products/nb110-58718_mouse-monoclonal-endoglin-cd105-antibody-mem-229-13120241638115.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]")
Applications for Endoglin/CD105 Antibody (MEM-229) - BSA Free
Application
Recommended Usage
Flow Cytometry
2-6 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Western Blot
1:1000
Application Notes
Western Blot: use non-reducing conditions. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Endoglin/CD105
Alternate Names
CD105, ENG
Gene Symbol
ENG
Additional Endoglin/CD105 Products
Product Documents for Endoglin/CD105 Antibody (MEM-229) - BSA Free
Product Specific Notices for Endoglin/CD105 Antibody (MEM-229) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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