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Endoglin/CD105 Antibody (MEM-229) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58718

Novus Biologicals, part of Bio-Techne
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NB110-58718

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine (Negative), Equine (Negative)

Cited:

Porcine

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-229

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

recombinant vaccinia virus containing human CD105 (L-isoform) cDNA

Specificity

This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Marker

Neo-endothelial Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endoglin/CD105 Antibody (MEM-229) - BSA Free

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PerCP-CyTM5.5.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) - BSA Free [NB110-58718]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT.

Applications for Endoglin/CD105 Antibody (MEM-229) - BSA Free

Application
Recommended Usage

Flow Cytometry

2-6 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Western Blot

1:1000
Application Notes
Western Blot: use non-reducing conditions. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Endoglin/CD105

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MEM-229) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Endoglin/CD105 Antibody (MEM-229) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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