Endoglin/CD105 Antibody (SN6)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65601
Clone SN6 was used by HLDA to establish CD designation.
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0004.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0008.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # SN6
Concentration
1 mg/ml
Product Summary for Endoglin/CD105 Antibody (SN6)
Immunogen
Partially purified cell membrane antigens from fresh leukemia cells
Reactivity Notes
Cross reacts with Horse, Cynomolgus Monkey, Rhesus Monkey, Monkey.
Specificity
Recognizes the human CD105 cell surface antigen, a glycoprotein homodimer of 95kD subunits. CD105 is also known as endoglin, and is expressed by endothelial cells, activated monocytes and some leukemia cells.
Marker
Neo-endothelial Cells Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Endoglin/CD105 Antibody (SN6)
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]
Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (SN6) NB100-65601PE and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.![Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0002.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]")
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]
Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488![Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0003.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]")
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]
Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647![Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0001.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0006.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]](https://resources.bio-techne.com/images/products/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0007.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]")
Applications for Endoglin/CD105 Antibody (SN6)
Application
Recommended Usage
Flow Cytometry
1:50
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Endoglin/CD105
Additional Endoglin/CD105 Products
Product Documents for Endoglin/CD105 Antibody (SN6)
Product Specific Notices for Endoglin/CD105 Antibody (SN6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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