Endophilin B1/Bif-1 Antibody (30A882.1.1) - Azide Free

Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1)Azide Free [NBP2-33258]

Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) - Azide Free [NBP2-33258] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) - Azide Free [NBP2-33258]

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) - Azide Free [NBP2-33258] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 5 ug/ml conc. of Endophilin B1 antibody (clone 30A882.1.1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Endophilin B1 was observed. Staining was performed by Histowiz. Image using the standard format of this product.

Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1)Azide Free [NBP2-33258]

Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) - Azide Free [NBP2-33258] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.