Endophilin B1/Bif-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-820

Novus Biologicals, part of Bio-Techne
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NB100-820
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Endophilin B1/Bif-1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QKGKVPITYLELLN corresponding to C-Terminus according to NP_057093.1.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Endophilin B1/Bif-1 Antibody

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820]

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820]

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of NIH3T3 (A), RAW264.7 (B) and KNRK (C) cell lysate with antibody at 0.1 ug/mL. RIPA buffer, 35 ug total protein per lane. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NB100-820]

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NB100-820]

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Endophilin B1/Bif-1 Antibody [NB100-820]

Flow Cytometry: Endophilin B1/Bif-1 Antibody [NB100-820]

Flow Cytometry: Endophilin B1/Bif-1 Antibody [NB100-820] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for Endophilin B1/Bif-1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 38 kDa band observed in lysates of human T-cell line Jurkat and in human lung and placenta (calculated MW of 40.8 kDa band according to NP_057093.1). A minor band of unknown identity was also consistently observed at 50 kDa band in Jurkat and placenta. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endophilin B1/Bif-1

Bif-1 is an evolutionarily conserved cytoplasmic protein that contains a predicted Src homology 3 (SH3) domain located near its C terminus but shares no significant homology with members of the Bcl-2 family. A Northern blot analysis indicates that Bif-1 is expressed in most tissues with abundant expression in heart and skeletal muscle. Bif-1 is capable of interacting with Bax as demonstrated by yeast two-hybrid, coimmunoprecipitation, and immunofluorescence studies. Induction of apoptosis in murine pre-B hematopoietic cells FL5.12 by interleukin-3 withdrawal results in increased association of Bax with Bif-1, which is accompanied by a conformational change in the Bax protein. Overexpression of Bif-1 promotes Bax conformational change, caspase activation, and apoptotic cell death in FL5.12 cells following interleukin-3 deprivation.

Long Name

SH3-domain GRB2-like Endophilin B1/Bax-interacting Factor 1

Alternate Names

Bif-1, Bif1, SH3GLB1

Entrez Gene IDs

51100 (Human); 54673 (Mouse)

Gene Symbol

SH3GLB1

UniProt

NP_057093 (Human)

Additional Endophilin B1/Bif-1 Products

Product Documents for Endophilin B1/Bif-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Endophilin B1/Bif-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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