Endophilin B1/Bif-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31130

Novus Biologicals, part of Bio-Techne
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NBP1-31130
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Endophilin B1/Bif-1 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Endophilin B1/Bif-1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (100%), Xenopus Tropicalis (100%).

Localization

Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Mitochondrion outer membrane, Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endophilin B1/Bif-1 Antibody

Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130]

Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130]

Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Endophilin B1/Bif-1 Antibody [NBP1-31130]

Immunocytochemistry/ Immunofluorescence: Endophilin B1/Bif-1 Antibody [NBP1-31130]

Immunocytochemistry/Immunofluorescence: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Endophilin B1/Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term (NBP1-31130).Blue: Hoechst 33342 staining.Scale bar = 10 um
Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130]

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130]

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bif-1 antibody (NBP1-31130).Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
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Applications for Endophilin B1/Bif-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endophilin B1/Bif-1

Bif, a member of the endophilin B protein family may be an important component of the mitochondrial pathway for apoptosis, as a novel Bax/Bak activator, and loss of this proapoptotic molecule may contribute to tumorigenesis. Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic protein via physical or chemical modifications of the membrane bilayer, particularly in presynaptic vesicle trafficking at nerve terminals. Bif (Endophilin B1), like endophilins A, binds to palmitoyl CoA, and exhibits lysophosphatidic acid acyl transferase activity.

Long Name

SH3-domain GRB2-like Endophilin B1/Bax-interacting Factor 1

Alternate Names

Bif-1, Bif1, SH3GLB1

Entrez Gene IDs

51100 (Human)

Gene Symbol

SH3GLB1

UniProt

Q9Y371

Additional Endophilin B1/Bif-1 Products

Product Documents for Endophilin B1/Bif-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endophilin B1/Bif-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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