Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05170
![Immunocytochemistry/ Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170]](https://resources.bio-techne.com/images/products/Endorepellin-Perlecan-Heparan-Sulfate-Proteoglycan-Antibody-A76-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05170-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Human
Applications
Validated:
ELISA, Immunoaffinity Purification, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # A76
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Specificity
Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170]
Immunocytochemistry/Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] - Perlecan (green) (G-I) secretion follows to stabilize the laminin and collagen IV networks. Scalebar = 20 um in all confocal micrographs. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fcell.2019.00224/full), licensed under a CC-BY license.
Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] -
Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] - Human primary brain microvascular endothelial cells stained for PDGF-BB (NBP1-58279), red and HSPG (NBP1-05170), green and nuclear DAPI blue. Image from a verified customer review.Applications
Application
Recommended Usage
ELISA
1:8000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 31750298). IHC: NBP1-05170 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP1-05170 is more sensitive than NBP2-23522 or NBP2-23523 in ELISA. (2) IP: NBP1-05170 can be used in immunoprecipitation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP1-05170 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Endorepellin/Perlecan
Long Name
Heparan Sulfate Proteoglycan
Alternate Names
HSPG2, Perlecan
Gene Symbol
HSPG2
Additional Endorepellin/Perlecan Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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