Endosialin/CD248/TEM1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77310
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1. The immunogen is located within the last 50 amino acids of TEM-1. Amino Acid Squence: GSKSPTEPMPPRGS
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)
Specificity
Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77310 can detect all isoforms of human, mouse and rat.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46, 81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Endosialin/CD248/TEM1 Antibody - BSA Free
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]
Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 4357, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310]
Immunohistochemistry: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Human Colon tissue with TEM1 antibody at 20 ug/mL.Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]
Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/mL.Applications for Endosialin/CD248/TEM1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
0.5-2 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30285896).
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Endosialin/CD248
Alternate Names
CD164L1, CD248, TEM1
Gene Symbol
CD248
UniProt
Additional Endosialin/CD248 Products
Product Documents for Endosialin/CD248/TEM1 Antibody - BSA Free
Product Specific Notices for Endosialin/CD248/TEM1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...