Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (93%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Endosialin/CD248 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TEM-1. The immunogen is located within amino acids 40-90 of TEM-1 Amino Acid Squence: ATPRTPEEAQRVDS

Specificity

Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD), and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and Rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77311 can detect human isoform 1, mouse and rat TEM-1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endosialin/CD248/TEM1 Antibody - BSA Free

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]

Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77311, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]

Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/ml.
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]

Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77310, (2 ug/mL), TEM-1 NBP1-77311, ( 1ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for Endosialin/CD248/TEM1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endosialin/CD248

Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion. At least two isoforms of TEM1 are known to exist; this antibody recognizes only the larger isoform.

Alternate Names

CD164L1, CD248, TEM1

Gene Symbol

CD248

UniProt

NP_065137

Additional Endosialin/CD248 Products

Product Documents for Endosialin/CD248/TEM1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endosialin/CD248/TEM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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