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Endosialin/CD248/TEM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14462

Novus Biologicals, part of Bio-Techne
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NBP2-14462-25ul
NBP2-14462

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: EEDEDEAWKAFNGGWTEMPGILWMEPTQPPDFALAYRPSFPEDREPQIPYPEPTWPPPLSAPRVPYHSSVLSVTRPVVVSATH

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26300407).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Endosialin/CD248/TEM1 Antibody

Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP2-14462]

Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP2-14462]

Immunocytochemistry/Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462]

Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462]

Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining in human placenta and tonsil tissues using anti-CD248 antibody. Corresponding CD248 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462]

Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462]

Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human placenta shows high expression.

Applications for Endosialin/CD248/TEM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endosialin/CD248

TEM1 may play a role in tumor angiogenesis

Alternate Names

CD164L1, CD248, TEM1

Gene Symbol

CD248

Additional Endosialin/CD248 Products

Product Documents for Endosialin/CD248/TEM1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Endosialin/CD248/TEM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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