Endothelial Lipase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB400-118
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative)
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
An N-terminal synthetic peptide made to human Endothelial Lipase [UniProt# Q9Y5X9]
Reactivity Notes
Does not appear to recognize mouse.
Localization
Secreted, cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Endothelial Lipase Antibody
Western Blot: Endothelial Lipase Antibody [NB400-118]
Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody [NB400-118]
Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-118] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Western Blot: Endothelial Lipase Antibody [NB400-118]
Western Blot: Endothelial Lipase Antibody [NB400-118] - Analysis of Endothelial Lipase in ECV304 cells.Applications for Endothelial Lipase Antibody
Application
Recommended Usage
ELISA
1:100-1:2000. Use reported in scientific literature (PMID 22383927)
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500
Application Notes
In Western blot this antibody recognizes a band at ~57 kDa, representing the mature, non-glycosylated form of endothelial lipase protein. Another band is seen at ~ 45 kDa, representing the proteolytic form of the protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Endothelial Lipase
Alternate Names
EC 3.1.1, EC 3.1.1.3, EDLendothelial lipase, ELlipoprotein lipase H, Endothelial cell-derived lipase, lipase, endothelial, PRO719
Gene Symbol
LIPG
UniProt
Additional Endothelial Lipase Products
Product Documents for Endothelial Lipase Antibody
Product Specific Notices for Endothelial Lipase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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