Endothelin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84779

Novus Biologicals, part of Bio-Techne
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NBP1-84779
NBP1-84779-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Endothelin-1 Antibodies »

Product Summary for Endothelin-1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PYGLGSPRSKRALENLLPTKATDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Endothelin-1 Antibody

Western Blot: Endothelin-1 Antibody [NBP1-84779]

Western Blot: Endothelin-1 Antibody [NBP1-84779]

Western Blot: Endothelin-1 Antibody [NBP1-84779] - Analysis in human plasma.
Immunocytochemistry/ Immunofluorescence: Endothelin-1 Antibody [NBP1-84779]

Immunocytochemistry/ Immunofluorescence: Endothelin-1 Antibody [NBP1-84779]

Immunocytochemistry/Immunofluorescence: Endothelin-1 Antibody [NBP1-84779] - Staining of human cell line A-431 shows localization to nucleus, nucleoli & the Golgi apparatus. Antibody staining is shown in green
Immunohistochemistry-Paraffin: Endothelin-1 Antibody [NBP1-84779]

Immunohistochemistry-Paraffin: Endothelin-1 Antibody [NBP1-84779]

Immunohistochemistry-Paraffin: Endothelin-1 Antibody [NBP1-84779] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells and endothelial cells.

Applications for Endothelin-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endothelin-1

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.

Alternate Names

ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME

Gene Symbol

EDN1

Additional Endothelin-1 Products

Product Documents for Endothelin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endothelin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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