Enolase 1 Antibody (2217)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43702

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (93%), Chicken (93%), Guinea Pig (95%), Mouse (94%), Primate (100%), Rhesus Macaque (100%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2217

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Enolase 1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Enolase 1. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Enolase 1 Antibody (2217)

Western Blot: Enolase 1 Antibody (2217) [NBP2-43702]

Western Blot: Enolase 1 Antibody (2217) [NBP2-43702]

Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: Enolase 1 Antibody (2217) [NBP2-43702]

Western Blot: Enolase 1 Antibody (2217) [NBP2-43702]

Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Analysis of A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract C. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [2217] dilution: 1:10000.
Western Blot: Enolase 1 Antibody (2217) [NBP2-43702]

Western Blot: Enolase 1 Antibody (2217) [NBP2-43702]

Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [2217].
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Applications for Enolase 1 Antibody (2217)

Application
Recommended Usage

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Enolase 1

Enolase 1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Alternate Names

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbol

ENO1

Additional Enolase 1 Products

Product Documents for Enolase 1 Antibody (2217)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Enolase 1 Antibody (2217)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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