Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15659

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15659-100ul
NBP3-15659-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9B4Y4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Enolase 2/Neuron-specific Enolase Antibodies »

Product Summary for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 47-100 of human Enolase 2/Neuron-specific Enolase (NP_001966.1). LELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [NBP3-15659]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [NBP3-15659]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (4D1Z6) [NBP3-15659] - Western blot analysis of extracts of various cell lines, using Enolase 2/Neuron-specific Enolase antibody (NBP3-15659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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