Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47635
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0001.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]")
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Western-Blot-NBP2-47635-img0003.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow (Intracellular), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # ENO2/1375
Concentration
0.2 mg/ml
Product Summary for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375)
Immunogen
A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104)
Localization
Cytoplasmic
Marker
Neuroendocrine Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54452)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375)
Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]
Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Analysis of Y79, HeLa and HepG2 Cell Lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0006.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]")
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - Formalin-fixed, paraffinembedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1375).![Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Flow-Intracellular-NBP2-47635-img0008.jpg "Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]")
Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]
Flow (Intracellular): Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635] - An intracellular stain was performed on HeLa cells with Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) NBP2-54452APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image from the Allophycocyanin version of this antibody.![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0004.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]](https://resources.bio-techne.com/images/products/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Immunohistochemistry-Paraffin-NBP2-47635-img0005.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) [NBP2-47635]")
Applications for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.2-0.4 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.2-0.4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Enolase 2/Neuron-specific Enolase
Additional Enolase 2/Neuron-specific Enolase Products
Product Documents for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375)
Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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