Enolase 2/Neuron-specific Enolase Antibody (SC06-28)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67641

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC06-28

Concentration

1 mg/ml

View all Enolase 2/Neuron-specific Enolase Antibodies »

Product Summary for Enolase 2/Neuron-specific Enolase Antibody (SC06-28)

Immunogen

Synthetic peptide within human Enolase 2/Neuron-specific Enolase aa 30-70. (SwissProt: P09104 Human; SwissProt: P17183 Mouse; SwissProt: P07323 Rat)

Localization

Cytoplasm, Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (SC06-28)

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Western blot analysis of Enolase 2/Neuron-specific Enolase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641]

Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641]

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SC06-28) [NBP2-67641] - Analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin.
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Applications for Enolase 2/Neuron-specific Enolase Antibody (SC06-28)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody (SC06-28)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (SC06-28)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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