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ENPP-3/CD203c Antibody (NP4D6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44643

Clone NP4D6 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-44643
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NP4D6

Format

BSA Free

Concentration

1 mg/ml

Product Summary for ENPP-3/CD203c Antibody (NP4D6) - BSA Free

Immunogen

HEK-293 cells transfected with human CD203c

Specificity

The mouse monoclonal antibody NP4D6 reacts with CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation. HLDA VIII

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ENPP-3/CD203c Antibody (NP4D6) - BSA Free

Immunocytochemistry/ Immunofluorescence: ENPP-3/CD203c Antibody (NP4D6) - BSA Free [NBP1-44643]

Immunocytochemistry/ Immunofluorescence: ENPP-3/CD203c Antibody (NP4D6) - BSA Free [NBP1-44643]

Immunocytochemistry/Immunofluorescence: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, red); merged signal yellow. DNA visualized by DAPI (blue).
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) - BSA Free [NBP1-44643]

Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) - BSA Free [NBP1-44643]

Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human peripheral blood by anti-CD203c antibody (NP4D6) FITC.
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) - BSA Free [NBP1-44643]

Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) - BSA Free [NBP1-44643]

Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by anti-CD203c antibody (NP4D6)

Applications for ENPP-3/CD203c Antibody (NP4D6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 - 5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ENPP-3/CD203c

CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 3

Alternate Names

B10, CD203c, ENPP3, NPP3, PD-IBETA, PDNP3

Gene Symbol

ENPP3

Additional ENPP-3/CD203c Products

Product Documents for ENPP-3/CD203c Antibody (NP4D6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ENPP-3/CD203c Antibody (NP4D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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