Do you have information as to what epitope these antibodies bind to (H00006036-D01P and H00006036-B01P)? Also, for developing an ELISA method, do you have a recommended concentration to use these antibodies at?

Name: Eosinophil derived neurotoxin Antibody
Brand: Novus Biologicals
SKU: H00006036-D01P

FYI, these two antibodies used the same immunogen (RNASE2 [NP_002925.1, 1 a.a. ~ 161 a.a] full-length human protein) to raise the polyclonal antibodies in rabbit (H00006036-D01P) and mouse (H00006036-B01P); and this is only different between them. Since they are polyclonal ones, the epitopes should cover the entire immunogens. Unfortunately, these two antibodies have never been tested together, as a pair, in sandwich-type ELISA (sELISA). They are more expected to work individually as the detection antibodies in ELISA. The users will need to test whether they can work together in the sELISA setting; apologize for any inconvenience. The datasheet for H00006036-B01P says that it is recommended to use dilution 1:100-1:2000 in ELISA. I anticipate that H00006036-D01P should use the similar dilution in ELISA too. In our experiences, sELISA has better to have a monoclonal antibody (or the antibody raised from a smaller immunogen) as the capture antibody, where a polyclonal (or the larger immunogen-derived antibody) as the detection one; so that the protein target is anchored to the surface by using the smaller region/less epitopes while the rest of epitopes can be used by the detection antibody.

Eosinophil derived neurotoxin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00006036-D01P

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

RNASE2 (NP_002925.1, 1 a.a. - 161 a.a.) full-length human protein. MVPKLFTSQICLLLLLGLLAVEGSLHVKPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTFLLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDNRDQRRDPPQYPVVPVHLDRII

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Eosinophil derived neurotoxin Antibody

Western Blot: Eosinophil derived neurotoxin Antibody [H00006036-D01P]

Western Blot: Eosinophil derived neurotoxin Antibody [H00006036-D01P] - Analysis of RNASE2 expression in human spleen.

Western Blot: Eosinophil derived neurotoxin Antibody [H00006036-D01P]

Western Blot: Eosinophil derived neurotoxin Antibody [H00006036-D01P] - Analysis of RNASE2 expression in transfected 293T cell line by RNASE2 polyclonal antibody.Lane 1: RNASE2 transfected lysate(18.40 KDa).Lane 2: Non-transfected lysate.

Applications for Eosinophil derived neurotoxin Antibody

Application

Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

It has been used for WB.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNase2/EDN

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities

Long Name

Eosinophil-Derived Neurotoxin

Alternate Names

RAF3, RNASE2, RNS2

Gene Symbol

RNASE2

UniProt

NP_002925.1 (Human)

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Product Documents for Eosinophil derived neurotoxin Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Eosinophil derived neurotoxin Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Eosinophil derived neurotoxin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00006036-D01P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all RNase2/EDN Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Scientific Data Images for Eosinophil derived neurotoxin Antibody

Western Blot: Eosinophil derived neurotoxin Antibody [H00006036-D01P]

Western Blot: Eosinophil derived neurotoxin Antibody [H00006036-D01P]

Applications for Eosinophil derived neurotoxin Antibody

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: RNase2/EDN

Long Name

Alternate Names

Gene Symbol

UniProt

Additional RNase2/EDN Products

Product Documents for Eosinophil derived neurotoxin Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Eosinophil derived neurotoxin Antibody