EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34274).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Flow Cytometry: EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free [NBP2-34532]

Flow Cytometry: EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free [NBP2-34532] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM/TROP1 Mouse Monoclonal Antibody (323/A3) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

EpCAM/TROP1 Antibody (323/A3) - (ECD)

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM/TROP1 antibody (323/A3) - (ECD). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

EpCAM/TROP1 Antibody (323/A3) - (ECD)

Formalin-fixed, paraffin-embedded human colon stained with EpCAM/TROP1 antibody (323/A3) - (ECD). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Western Blot

0.25-0.5ug/ml

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot (Non-reducing only): 0.5-1.0ug/ml.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: EpCAM/TROP1

Epithelial cell adhesion molecule (EpCAM), also known as TROP1 and CD326, is a type I transmembrane glycoprotein that plays a role in a number of cellular processes including adhesion, signaling, maintaining stemness, proliferation, migration, and invasion (1,2). The human EpCAM protein is 314 amino acids (aa) in length with a theoretical molecular weight (MW) of ~35 kDa (1,3,4). The EpCAM protein includes a signal peptide, an extracellular N-terminal domain, a thyroglobulin type-1 domain, a carboxyl-terminal domain, a single-pass transmembrane domain, and an intracellular domain (1,3). The protein is highly conserved and has approximately 81% aa sequence identity between human and mouse (5). Trophoblast cell-surface antigen 2 (TROP2) also shares ~67% aa sequence similarity with EpCAM (1,5). While both EpCAM and TROP2 are cell surface markers expressed in the epithelium, their expression levels typically do not correlate (5). EpCAM is expressed in normal epithelial tissue and elevated expression is observed in many epithelial carcinomas (1-3) Patient tumor samples with increased EpCAM expression have been associated with poor prognosis (1). Given EpCAM's elevated expression in tumors, it has become a potential target for cancer therapy approaches, including monoclonal antibody treatment and anti-EpCAM trispecific antibodies (1,5).

EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).

References

1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255

2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764

3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956

4. Uniprot (P16422)

5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Gene Symbol

EPCAM

Additional EpCAM/TROP1 Products

Product Documents for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34532

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Format

Concentration

View all EpCAM/TROP1 Antibodies »

Product Summary for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Immunogen

Reactivity Notes

Localization

Specificity

Marker

Clonality

Host

Isotype

Description

Scientific Data Images for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Flow Cytometry: EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free [NBP2-34532]

EpCAM/TROP1 Antibody (323/A3) - (ECD)

EpCAM/TROP1 Antibody (323/A3) - (ECD)

Applications for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: EpCAM/TROP1

Long Name

Alternate Names

Gene Symbol

Additional EpCAM/TROP1 Products

Product Documents for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for EpCAM/TROP1 Antibody (323/A3) - (ECD) - Azide and BSA Free