EPCR Antibody (RCR-252) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-963

Clone RCR-252 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, Flow Cytometry, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # RCR-252

Format

BSA Free

Concentration

2.4 mg/ml

View all EPCR Antibodies »

Product Specifications

Immunogen

Myeloma cells and cells isolated from the superficial inguinal lymph nodes from Wistar rats immunized with the human EPCR-positive RE-1 cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EPCR Antibody (RCR-252) - BSA Free

Flow Cytometry: EPCR Antibody (RCR-252) - BSA Free [NB600-963]

Flow Cytometry: EPCR Antibody (RCR-252) - BSA Free [NB600-963]

Flow Cytometry: EPCR Antibody (RCR-252) [NB600-963] - 1x10 HUVEC cells were labled with u Ug/test Anti-Enothelial cell protein C Receptor antibody, rat monoclonal RCR-252.

Applications for EPCR Antibody (RCR-252) - BSA Free

Application
Recommended Usage

Flow Cytometry

5-20 ug/ml
Application Notes
Blocks the binding of the APC ligand to the EPCR.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

2.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EPCR

Blood coagulation depends on a series of sequential proteolytic processes, leading to the generation of new serine proteases. Several natural anticoagulant mechanisms are balancing the clotting system. One of them is the protein C anticoagulant pathway. In this pathway, thrombin binds to the endothelial cell receptor thrombomodulin. This complex activates protein C to generate the anticoagulant enzyme activated protein C (APC) that when complexed to protein S inhibits coagulation by inactivating two critical regulatory proteins: factor Va and VIIIa.3 Protein C activation by the thrombin/ thrombomodulin complex is a relatively low-affinity reaction. However, it was found that protein C and APC bind to culture endothelial cells with a relatively high affinity. The high affinity binding of both ligands (Kd = 30nM) is mediated by EPCR (endothelial cell protein C receptor). EPCR is a type 1 transmembrane glycoprotein containing two domains in the extra cellular region that are homologous to the alpha 1 and alpha 2 domains of CD1/MHC class 1 molecules.4 The mature human EPCR protein contains 211 amino acids. The protein migrates as a 49 kDa protein due to its carbohydrates chains.1 The protein is expressed in endothelial cells present in various types of vessels including arterial heart, lung, and in the vena cava inferior. The EPCR is also expressed in small vessels such as capillaries of the alveolar wall in the lung. The high-affinity binding of protein C to EPCR is a critical step for activation. Upon binding, the structure of protein C is modulated to enable rapid conversion to the active form. This enhances the activation rate by at least 5-fold.3, 4 The EPCR protein appears to perform other functions, as well. It binds proteinase 3. The complex then binds to MAC-1 on activated neutrophils and inhibits their adhesion to activated endothelium. Another property of EPCR is that it can translocate from the membrane to the nucleus. The RCR-252 monoclonal antibody strongly inhibits the interaction of APC with EPCR. The region recognized by the RCR-252 antibody is critical for binding of APC.2 Antibodies, which react specifically with the EPCR protein and inhibit ligands binding to it, are an essential tool for exploring the physiological effects of protein C and APC binding on the mechanisms of blood clotting in homeostasis and various thrombotic disorders.

Long Name

Endothelial Protein C Receptor

Alternate Names

CCD41, CD201, PROCR

Entrez Gene IDs

10544 (Human)

Gene Symbol

PROCR

Additional EPCR Products

Product Documents for EPCR Antibody (RCR-252) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EPCR Antibody (RCR-252) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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