EphA1 Antibody (5D2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1763

Novus Biologicals, part of Bio-Techne
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NB100-1763
NB100-1763-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D2

Format

BSA Free

Concentration

1 mg/ml

View all EphA1 Antibodies »

Product Summary for EphA1 Antibody (5D2) - BSA Free

Immunogen

Purified recombinant fragment of EphA1 expressed in E. Coli.

Specificity

EPH Receptor A1 (5D2D1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EphA1 Antibody (5D2) - BSA Free

Western Blot: EphA1 Antibody (5D2)BSA Free [NB100-1763]

Western Blot: EphA1 Antibody (5D2)BSA Free [NB100-1763]

Western Blot: EphA1 Antibody (5D2D1) [NB100-1763] - Analysis using EphA1 mouse mAb against A549 (1) and Hela (2) cell lysate.
Immunohistochemistry-Paraffin: EphA1 Antibody (5D2) - BSA Free [NB100-1763]

Immunohistochemistry-Paraffin: EphA1 Antibody (5D2) - BSA Free [NB100-1763]

Immunohistochemistry-Paraffin: EphA1 Antibody (5D2D1) [NB100-1763] - Analysis of human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic localization using EphA1 mouse mAb with DAB staining.

Applications for EphA1 Antibody (5D2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphA1

EPH receptor A1 (EphA1), with 976-amino acid protein(about 107 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date and their ligands, the ephrins,can be subdivided into two major subclasses, ephrin-A and ephrin-B. Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior, particularly in the nervous system. They have been implicated in various developmental processes, including axonal guidance, angiogenesis, morphogenesis and carcinogenesis.

Alternate Names

Esk

Entrez Gene IDs

2041 (Human)

Gene Symbol

EPHA1

Additional EphA1 Products

Product Documents for EphA1 Antibody (5D2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EphA1 Antibody (5D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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