EphA2 Antibody (1C1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81069

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 kappa Clone # 1C1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all EphA2 Antibodies »

Product Specifications

Immunogen

1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.

Specificity

1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.

Clonality

Monoclonal

Host

Human

Isotype

IgG1 kappa

Scientific Data Images for EphA2 Antibody (1C1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: EphA2 Antibody (1C1) - Azide and BSA Free [NBP2-81069]

Immunocytochemistry/ Immunofluorescence: EphA2 Antibody (1C1) - Azide and BSA Free [NBP2-81069]

Immunocytochemistry/Immunofluorescence: EphA2 Antibody (1C1) [NBP2-81069] - Live murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 at 2 ug/ml and a donkey anti-mouse IgG-AF488 at 20 ug/ml for 90 mins. Nuclei stained with Hoechst. Cells captured at 20x magnification. Panel A shows the AF488 signal, panel B the DIC image and panel C a merge of fluorescence (AF488 + Hoechst) with the DIC image. In this live sample, antibody internalisation is observed.
Immunocytochemistry/ Immunofluorescence: EphA2 Antibody (1C1) - Azide and BSA Free [NBP2-81069]

Immunocytochemistry/ Immunofluorescence: EphA2 Antibody (1C1) - Azide and BSA Free [NBP2-81069]

Immunocytochemistry/Immunofluorescence: EphA2 Antibody (1C1) [NBP2-81069] - Fixed murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 at 2 ug/ml and a donkey anti-mouse IgG-AF488 at 20 ug/ml for 90 mins. Nuclei stained with Hoechst. Cells captured at 20x magnification. Panel A shows a merge of the fluorescence channels, whilst panel B shows the DIC image. In this fixed sample the cell membrane is clearly outlined.

Applications for EphA2 Antibody (1C1) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: EphA2

Eph receptor A2 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

Alternate Names

Eck, Myk2, Sek2

Gene Symbol

EPHA2

Additional EphA2 Products

Product Documents for EphA2 Antibody (1C1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EphA2 Antibody (1C1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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