EphA3 Antibody (3A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00002042-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EphA3 Antibodies »

Product Specifications

Immunogen

EPHA3 (AAH63282, 202 a.a. ~ 326 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACRPGFYKALDGNMKCAKCPPHSSTQEDGSMNCRCENNYFRADKDPPSMACTRPP

Specificity

EPHA3 - EPH receptor A3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EphA3 Antibody (3A12)

Western Blot: EphA3 Antibody (3A12) [H00002042-M01]

Western Blot: EphA3 Antibody (3A12) [H00002042-M01]

Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - EPHA3 monoclonal antibody (M01), clone 3A12. Analysis of EPHA3 expression in human spleen.
Western Blot: EphA3 Antibody (3A12) [H00002042-M01]

Western Blot: EphA3 Antibody (3A12) [H00002042-M01]

Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - analysis of EPHA3 expression in transfected 293T cell line by EPHA3 monoclonal antibody (M01), clone 3A12. Lane 1: EPHA3 transfected lysate (110.1 KDa). Lane 2: Non-transfected lysate.
Western Blot: EphA3 Antibody (3A12) [H00002042-M01]

Western Blot: EphA3 Antibody (3A12) [H00002042-M01]

Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - Analysis of EPHA3 over-expressed 293 cell line, cotransfected with EPHA3 Validated Chimera RNAi ( Cat # H00002042-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EPHA3 monoclonal antibody (M01) clone 3A12 (Cat # H00002042-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for EphA3 Antibody (3A12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EphA3

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Long Name

Eph Receptor A3

Alternate Names

Cek4, Hek4, Tyro4

Entrez Gene IDs

2042 (Human)

Gene Symbol

EPHA3

OMIM

179611 (Human)

UniProt

AAH63282 (Human)

Additional EphA3 Products

Product Documents for EphA3 Antibody (3A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EphA3 Antibody (3A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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