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EphA4 Antibody (6H7)

Novus Biologicals, part of Bio-Techne | Catalog # H00002043-M02

Novus Biologicals, part of Bio-Techne
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H00002043-M02

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EPHA4 (NP_004429, 887 a.a. ~ 986 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SLKRTGTESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

EPHA4 (6H7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EphA4 Antibody (6H7)

Western Blot: EphA4 Antibody (6H7) [H00002043-M02]

Western Blot: EphA4 Antibody (6H7) [H00002043-M02]

Western Blot: EphA4 Antibody (6H7) [H00002043-M02] - EPHA4 monoclonal antibody (M02), clone 6H7 Analysis of EPHA4 expression in NIH/3T3.
Sandwich ELISA: EphA4 Antibody (6H7) [H00002043-M02] - Detection limit for recombinant GST tagged EPHA4 is approximately 0.1ng/ml as a capture antibody.

Applications for EphA4 Antibody (6H7)

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EphA4

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Long Name

Eph Receptor A4

Alternate Names

Cek8, Hek8, Sek1, Tyro1

Entrez Gene IDs

2043 (Human)

Gene Symbol

EPHA4

OMIM

602188 (Human)

UniProt

Additional EphA4 Products

Product Documents for EphA4 Antibody (6H7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EphA4 Antibody (6H7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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