Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 86731

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EphA5 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat EphA5
Ser58-Gln573 (Asp170Glu)
Accession # P54757

Specificity

Detects rat EphA5 in direct ELISAs and Western blots. In direct ELISAs, 5‑15% cross reactivity with recombinant mouse (rm) EphA4 and rmEpha8 is observed and no cross-reactivity with recombinant human (rh) EphA1, rmEphA2, -A3, -A6, -A7, recombinant rat EphB1, -B2, or rmEphB3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for EphA5 Antibody (86731) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: EphA5

The EPHA5 gene encodes a member of a subfamily of the protein-tyrosine kinase family, an ephrin type-A receptor 5 receptor that exists in three isoforms: isoform 1: 1,037 amino acids long at 114 kDA; isoform 2: 1,015 amino acids long, 112 kDA; and isoform 3: 969 amino acids long at 107 kDA. Receptors of this family function in mediating developmental events especially in the nervous system. EPHA5 participates in the JNK pathway, P2Y receptor signaling, paxillin interactions, intracellular calcium signaling, and EphrinA-ephR signaling. It interacts with genes EFNA2, EFNA1, EFFNA3, EFNA5, and STAT3. EPHA4 is linked to pancreatitis, glioblastoma, cerebritis, ovarian cancer, adenocarcinoma, breast cancer and drug psychosis.

Alternate Names

Bsk, Cek7, Ehk1, Hek7, Rek7

Gene Symbol

EPHA5

Additional EphA5 Products

Product Documents for EphA5 Antibody (86731) [PE/Cy7]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EphA5 Antibody (86731) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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