EphB1 Antibody (SR1093)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21984

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21984-100ul
NBP3-21984-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1093

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EphB1 Antibodies »

Product Summary for EphB1 Antibody (SR1093)

Immunogen

A synthesized peptide derived from human EphB1 (Uniprot #: P54762)

Specificity

Detects endogenous levels of total EphB1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EphB1 Antibody (SR1093)

Western Blot: EphB1 Antibody (SR1093) [NBP3-21984] -

Western Blot: EphB1 Antibody (SR1093) [NBP3-21984] - Western blot analysis using NBP3-21984 in U87MG cell lysate.

Applications for EphB1 Antibody (SR1093)

Application
Recommended Usage

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphB1

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

Long Name

Eph Receptor B1

Alternate Names

Cek6, Elk, Hek6, Net

Gene Symbol

EPHB1

Additional EphB1 Products

Product Documents for EphB1 Antibody (SR1093)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EphB1 Antibody (SR1093)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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