EphB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55398

Novus Biologicals, part of Bio-Techne
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NB100-55398
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all EphB2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QKDRNHRPKFGQ corresponding to internal region according to NP_004433.2, NP_059145.2.

Epitope

QKDRNHRPKFGQ

Specificity

This antibody is expected to recognise both reported isoforms ( NP_059145.2 and NP_004433.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EphB2 Antibody

Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398] - Analysis of Human lung and placenta lysates using NB100-55398.
Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398] - (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for EphB2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human lung and placenta lysates is reported in scientific literature (PMID: 17428795).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EphB2

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Long Name

Eph Receptor B2

Alternate Names

Cek5, Drt, Erk, Hek5, Nuk, Qek2, Sek3, Tyro5

Entrez Gene IDs

2048 (Human); 13844 (Mouse)

Gene Symbol

EPHB2

UniProt

NP_004433.2 (Human)

Additional EphB2 Products

Product Documents for EphB2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EphB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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