Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 647354

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EphB3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EphB3
Leu38-Ala550
Accession # P54753

Specificity

Detects human EphB3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) EphA3, rhEphA4, EphB2, or recombinant mouse EphB3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for EphB3 Antibody (647354) [PE/Atto594]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: EphB3

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Long Name

Eph Receptor B3

Alternate Names

Cek10, Hek2, Mdk5, Sek4, Tyro6

Gene Symbol

EPHB3

Additional EphB3 Products

Product Documents for EphB3 Antibody (647354) [PE/Atto594]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EphB3 Antibody (647354) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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