Ephrin-A1 Antibody (A7B10-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32312

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32312
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # A7B10-R

Concentration

1 mg/ml

View all Ephrin-A1 Antibodies »

Product Summary for Ephrin-A1 Antibody (A7B10-R)

Immunogen

Recombinant protein within Human Ephrin-A1 aa 13-190 / 205. (Uniprot: P20827)

Localization

Cell membrane, Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ephrin-A1 Antibody (A7B10-R)

Ephrin-A1 Antibody (A7B10-R)

Western Blot: Ephrin-A1 Antibody (A7B10-R) [NBP3-32312]

Western Blot: Ephrin-A1 Antibody (A7B10-R) [NBP3-32312] - Western blot analysis of Ephrin-A1 on HUVEC cell lysates with Mouse anti-Ephrin-A1 antibody (NBP3-32312) at 1/2,000 dilution.

Lysates/proteins at 20 ug/Lane.

Predicted band size: 24 kDa
Observed band size: 25 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32312) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Applications for Ephrin-A1 Antibody (A7B10-R)

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ephrin-A1

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

Alternate Names

B61, EFL-1, EFNA1, EphrinA1, LERK1

Gene Symbol

EFNA1

Additional Ephrin-A1 Products

Product Documents for Ephrin-A1 Antibody (A7B10-R)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ephrin-A1 Antibody (A7B10-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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