Ephrin-A2 Antibody (OTI3E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02810
![Flow Cytometry: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]](https://resources.bio-techne.com/images/products/Ephrin-A2-Antibody-3E3-Flow-Cytometry-NBP2-02810-img0001.jpg "Flow Cytometry: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]")
![Western Blot: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]](https://resources.bio-techne.com/images/products/Ephrin-A2-Antibody-3E3-Western-Blot-NBP2-02810-img0002.jpg "Western Blot: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3E3
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ephrin-A2 Antibody (OTI3E3)
Western Blot: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]
Western Blot: Ephrin A2 Antibody (3E3) [NBP2-02810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ephrin A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ephrin A2.
Immunocytochemistry/Immunofluorescence: Ephrin A2 Antibody (3E3) [NBP2-02810] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.
![Immunohistochemistry-Paraffin: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]](https://resources.bio-techne.com/images/products/Ephrin-A2-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-02810-img0003.jpg "Immunohistochemistry-Paraffin: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]")
Immunohistochemistry-Paraffin: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]
Immunohistochemistry-Paraffin: Ephrin A2 Antibody (3E3) [NBP2-02810] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ephrin A2 mouse monoclonal antibody.Applications for Ephrin-A2 Antibody (OTI3E3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ephrin-A2
Alternate Names
Cek7-L, EFNA2, ELF-1, EphrinA2, LERK6
Entrez Gene IDs
1943 (Human)
Gene Symbol
EFNA2
Additional Ephrin-A2 Products
Product Documents for Ephrin-A2 Antibody (OTI3E3)
Product Specific Notices for Ephrin-A2 Antibody (OTI3E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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