Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70623
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]](https://resources.bio-techne.com/images/products/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0003.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]")
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]](https://resources.bio-techne.com/images/products/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0006.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI4A4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 23-214 of human EFNA3(NP_004943) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
26.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]](https://resources.bio-techne.com/images/products/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0001.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]")
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]](https://resources.bio-techne.com/images/products/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0002.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]")
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]](https://resources.bio-techne.com/images/products/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0004.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]")
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]](https://resources.bio-techne.com/images/products/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0005.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]")
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]](https://resources.bio-techne.com/images/products/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0007.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]")
Applications for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ephrin-A3
Alternate Names
EFL-2, EFNA3, Ehk1-L, EphrinA3, LERK3
Gene Symbol
EFNA3
Additional Ephrin-A3 Products
Product Documents for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free
Product Specific Notices for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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